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15th April 2019 - Spray Drying Process Development and Scale up

Upperton Pharma Solutions are one of the very few CDMO’s that have a bespoke, fully equipped non-GMP pilot plant designed to reduce the timelines and the costs for scale up and transfer of spray drying processes into GMP.

Spray Drying Development and Scale up

Formulation and early process development studies are performed in the Upperton spray drying development suite on one of our laboratory-scale Büchi B290 spray dryers. 

These are excellent spray dryers for early development studies; enabling us to produce stable formulations to meet our customers' requirements. They also allow us to define the process design space for producing the spray dried formulation using a DoE approach. 

The next step towards clinical manufacture is transfer of the process onto one of the GMP-compliant spray dryers, housed in our non-GMP pilot plant. 

Depending upon the scale eventually required for clinical manufacture, the process may be transferred onto our ProCepT 4M8-TriX spray dryer or it may be scaled up onto our GEA Niro Mobile Minor spray dryer which can be used to produce batches of up to ~3kg spray dried product. 

The Upperton pilot plant is equipped with exactly the same spray dryers as our GMP facility. This allows us to produce kilogram quantities of non-GMP material to support toxicology and stability studies at a fraction of the cost of GMP manufacture. 

Once tech transfer / scale up studies are completed: A Master Batch Record can be produced, Chemistry, Manufacturing and Controls (CMC) batches can be manufactured and the process is ready to be transferred directly into the GMP facility for clinical manufacture. 

19th March 2019 - Engineered Powders for Enhanced Pulmonary Delivery

Engineered Powders for Enhanced Pulmonary Delivery

Upperton is continuing to invest in its combined spray drying and micronisation technology in order to meet increased demand. This emerging processing technology has been developed to enable the production of larger quantities of dry powder formulations suitable for pulmonary delivery.

The expansion of this service has been driven by growing demand for this exciting next generation pulmonary delivery technology particularly from customers requiring GMP manufacturing to support clinical trials.

Producing dry powders with the optimum aerodynamic particle size distribution of 1-5 µm for pulmonary delivery is fairly straightforward on a laboratory scale spray dryer. Feedstocks of low solids concentration are typical; processing rates are kept relatively low and high atomisation gas pressures are employed in order to generate the very small particles needed for high levels of deposition and uptake in the alveoli.

Challenges arise when larger quantities of material are required and a scaled-up, commercially viable process is essential.  Particles in the 1-5 µm range are notoriously difficult to produce and collect efficiently on larger pilot or commercial scale spray dryers. Size distributions can often creep upwards, and product yields can plummet. This is particularly bad news for high value APIs!

The Upperton approach is to optimise the spray drying process for product yield, with particle size no longer the primary focus. The feedstock concentration and processing rate can both be increased; significantly reducing both manufacturing time and cost. The spray dried particles are then reduced to the target particle size distribution, using a carefully controlled dry powder milling process. The result is a much faster, more efficient process producing large quantities of powders with excellent aerolisation properties.
 
Impaction testing of our model pulmonary formulations shows that we can reproducibly generate dry powders with an aerodynamic particle size distribution of 1-5 µm, with fine particle fraction in excess of 70% when formulated powders are delivered from commercially available DPI’s.

5th March 2019 - Analytical Techniques for Biotherapeutics.

Analysing Biotherapeutic Formulations

There is growing interest in the use of spray drying to create biotherapeutic dry powder formulations. Spray drying is becoming increasingly viewed as a more cost-effective method for enhancing the stability of thermo-sensitive molecules (such as proteins, peptides, monoclonal antibodies and vaccines).
 
A key aspect in the successful development of dry powder formulations is identifying suitable analytical techniques to evaluate the biologic in question. These tests will be needed to: aid formulation development, determine the impact of different spray drying parameters on the product and to determine stability profile. There are a wide range of assays available so it is important to choose suitable assays that can provide insight into two important aspects of the final powders produced, namely assays that show:  

  • The biologic in question retains its structure and activity both during the spray drying process and on subsequent storage.

  • The spray drying process is generating powders with the necessary physical properties required for powder handling, reconstitution and storage stability.

There is a range of potential assays that can be used to evaluate the spray dried formulation, the list is extensive therefore,some of the key tests that practitioners might want to consider have been highlighted. The assays have been divided into two groups: those used to evaluate the physical properties of the spray dried powders and those assays designed to evaluate the structure, function and chemical properties of the biologic itself.

Physical properties of the spray dried powder

Test

Rationale

Particle size analysis
(Laser light scattering, impaction by ACI/NGI)

Particle sizing will give an insight into powder handling requirements, storage stability and performance in delivery devices such as dry powder inhalers.

Residual moisture
(loss on drying, Karl Fischer)

Tight control of residual moisture is an important read out in the optimisation of spray drying conditions and tight specification will be needed in order to meet storage stability targets.

Thermal properties
DSC/DVS)

Thermal properties, such as moisture uptake, melting points or glass transition temperatures of the powders produced will impact on handling and storage stability.

Powder flow properties
(Bulk/tapped density, angle of repose)

One of the major challenges of spray drying is engineering powders that have suitable handling and flow characteristics.

Electron microscopy
(scanning, transmission)

Important for evaluating particle morphology, evidence of any crystallinity and retention of physical structure of biologic (e.g. virosomes).

 

Structure, function and chemical properties of the biologic

Test

Rationale

HPLC
analysis (size exclusion, reverse phase)

Established technique for evaluating aggregation of biologic molecules (during spray drying and on storage) and for evaluating chemical modification(s) of specific amino acids.

Gel electrophoresis
(denaturing/non-denaturing, Western blotting)

Technique for evaluating covalent and non-covalent molecular weight changes of structural components (including aggregation, dissociation and scission).

Biologic functional assays
(e.g. enzyme activity, antigenicity, ELISA)

Testing the activity of the molecule being formulated is an important test of the molecule's integrity during spray drying and on subsequent storage.

Mass spectroscopy

Sensitive technique especially useful for detecting oxidation and deamidation of amino acid side chains that can occur during spray drying and on storage.

Spectrophotometric assays
(reconstituted powder)

Assess reconstitution time, presence of insoluble material (aggregation), substrate binding studies.

Circular Dichroism

Used to determine protein folding, looking at secondary and tertiary structures.

 

19th February 2019 - Biotherapeutic Formulation Development

Spray Drying Biotherapeutics

Dry powder formulations of Biotherapeutics eliminate the need for expensive cold chain storage and facilitate supply chain management. Lyophilisation is traditionally used for the drying process, but in recent years the semi-continuous, robust and scalable qualities of spray drying have made it the drying process of choice.

With expert formulation and process development, even the most delicate biomolecule can be spray dried with little or no loss of activity.

To understand this, it is useful to consider the spray drying process: The solution is first pumped into the drying chamber through a two-fluid nozzle, at the tip of which it is atomised into droplets by a flow of compressed air. The design of the nozzle means that the cool atomisation air protects the liquid from the high temperature of the drying gas right up until the point of atomisation. Once the solution is atomised, the droplets dry very quickly in the heated drying airstream; again, the biomolecule is protected from the heat by an evaporative-cooling effect as the droplets dry. Once in the dry form, biomolecules are generally much more robust than in solution, and the total residence time within the spray dryer is only a matter of seconds, depending upon the size of the dryer.

 

Biotherapeutic Formulation Development

Formulation development is important to ensure the stability of biomolecules at three critical points during and after processing: on solution atomisation, during the drying process, and on storage after drying.

Surfactants, such as polysorbates or poloxamers, offer protection at the liquid-air interface during the atomisation stage, preventing protein aggregation or denaturation.

Stabilisation during the drying process is achieved by the use of excipients that can form hydrogen-bonds with the biomolecule. Sugars such as trehalose, raffinose, or dextran are commonly used, due to their high glass transition temperatures; offering enhanced storage stability of the bioformulations. Combinations of amino acids such as histidine, glycine, proline and arginine, can be tailored to offer effective stabilisation; resulting in dry powder bioformulations with excellent stability characteristics.

Polymers, for example: cyclodextrin, PVP, methyl-cellulose and eudragits may be used in bioformulations, both for enhanced stability and for other purposes (controlled release, taste masking and enteric coating).

Other commonly used excipients include:

·          Salts for increasing ionic strength of solution, minimising electrostatic interactions, and control of pH

·          Antioxidants

·          Divalent metal ions, such as zinc, to form dimers and reduce protein aggregation

·          Amino acids, such as Leucine, and surfactants such as Phophatidylcholine, for improved powder flow and aerosolization

·          Mucoadhesives such as chitosan in formulations for nasal delivery

 

Process Development

Spray drying processing parameters can have a profound effect on the properties of dry powder bioformulations, and the process development phase is of critical importance.

In optimising the drying temperature, a balance must often be struck between the need for a low residual moisture level in the product for optimum storage stability, and a drying temperature that the biomolecule can withstand. The drying temperature can also affect the stickiness of the product, depending upon the glass transition temperature of the formulation.

Particle size and morphology have an impact on powder flow and aerosolisation properties, which are especially important in formulations for inhaled or nasal delivery. These can be controlled by optimisation of a range of process parameters: Feed solution concentration, liquid feed rate, atomisation air pressure and air flow rate, have the biggest impact on particle size. Drying air flow rate and temperature affect the rate of drying and so can be used to change the morphology of the dried particles.

 

Characterisation of Spray Dried Bioformulations

Upperton has a wide range of analytical techniques that can be used to investigate the chemical and physical properties of dry powder bioformulations. These include HPLC, gel electrophoresis, activity assays, particle size analysis by laser diffraction and impaction (ACI/NGI), residual moisture determination, differential scanning calorimetry, dynamic vapour sorption, powder flow measurements, and scanning electron microscopy.

5th February 2019 - Extended Capabilities on Upperton's non-GMP Pilot Plant

Upperton Pharma Solutions now has a fully commissioned 60 square metre non-GMP pilot plant, mirroring our GMP manufacturing facility. The pilot plant is equipped for three scales of spray drying:

           ProCept 4M8-Trix for batch sizes of up to 300g

           Upperton Intermediate Spray Dryer for batch sizes of up to 1kg

           GEA Niro Mobile Minor for batch sizes of up to 3kg

In addition, the pilot plant has all of the powder handling and processing capabilities offered in our GMP manufacturing facility, for downstream processing of spray dried powders into final dosage forms.

Formulations and processes optimised in our R&D laboratories can be quickly scaled up to produce non-GMP material for toxicology and stability studies and CMC batches. The mirroring of our GMP capability enables a rapid seamless transfer of processes into clinical manufacture, along with all of the expertise and formulation-specific know-how built up during the development stages.

22nd January 2019 - Successful completion of EU Horizon 2020 Grant MACIVIVA

In 2014, Upperton Pharma Solutions was awarded a prestigious Horizon 2020 grant as part of a European consortium led by Mymetics, a pioneering company at the forefront of the development of virosome-based vaccines.

The MACIVIVA goal was to develop dry powder, thermostable, cold-chain independent virosomal vaccine formulations for non-invasive needle-free administration. Such solid form vaccines should reduce the cost and improve safety and compliance in National Immunisation Programs, particularly in the developing world.

The Upperton formulation development team successfully developed spray dried formulations of HIV virosomal vaccines for both oral and nasal delivery routes. The optimised processes were scaled up and transferred into the company’s GMP clean room facility, where batches of both the Oral and the Nasal formulation were manufactured.

Upperton’s spray dried Virosomal Vaccine formulations:

  • Retained virosome structure and particle size on spray drying
  • Preserved antigenicity in the final solid vaccine forms
  • Retained the initial vaccine immunogenicity after storage for three months at 40°C / 75%RH
  • Both nasal and oral formulations were successfully dosed in animal models
  • The immunogenicity of the nasal solid vaccine form was comparable to subcutaneous injection of the reference liquid vaccine

Participation in the MACIVIVA project has enabled Upperton to expand their technical capabilities to include GMP manufacture of solid form virosomal vaccines.

Successful Completion of N2 Business Growth Grant

In October 2017, Upperton was delighted to be awarded grant funding by the Nottinghamshire-based N2 Business Growth Fund to support investment into a capital project leading to business growth and the creation of new sustainable jobs. Upperton used the funds to support the establishment of a GMP clinical manufacturing capability at its Nottingham site.

The success of the project was easily measured: In November 2018 Upperton Pharma Solutions’ manufacturing facilities passed inspection by the UK Medicines and Healthcare products Regulatory Agency. The MHRA licence allows the company to develop pharmaceutical formulations for oral, nasal and pulmonary delivery right through from early feasibility studies to scale up and Phase I & II clinical manufacture, all from its Nottingham Science and Technology Park site.

8th January 2019 - MHRA Inspection success for Nottingham’s Upperton Pharma Solutions

Upperton Pharma Solutions’ new pharmaceutical spray drying manufacturing facilities at Nottingham Science and Technology Park have successfully passed inspection by the UK Medicines and Healthcare products Regulatory Agency (MHRA).

A pharmaceutical spray drying contract development and manufacturing organisation (CDMO), Upperton Pharma Solutions moved to its new facilities on Nottingham Science and Technology Park from its previous headquarters at BioCity Nottingham in March 2018, as part of a planned expansion of its services.

The company has invested heavily in the new facilities both in terms of building enhancements and in the expansion of its processing and analytical capabilities.

The successful MHRA inspection provides Upperton Pharma Solutions with the necessary MHRA licenses for manufacture, downstream processing into capsules and tablets, as well as release of spray dried formulations of Biotherapeutics and small molecule APIs for clinical trials.

The company is now able to develop pharmaceutical formulations for oral, nasal and pulmonary delivery from early feasibility studies to Phase I & II clinical manufacture, from its Nottingham Science and Technology Park site.

Richard Johnson, CEO and Founder of Upperton Pharma Solutions, said: “We are absolutely delighted with the expansion of our capabilities in GMP clinical manufacturing and look forward to seeing our clients’ projects progress within Upperton, from early formulation development studies right through to clinical manufacture.”

“The positive endorsement by the MHRA will enable a seamless transition from R&D to GMP manufacture within the organisation, ensuring that all of the project-specific knowledge and expertise developed in R&D will flow through to the GMP manufacturing processes.”

Paul Kelsall, Director of Clinical Trials Manufacturing commented: “This is a great opportunity for our business and is a result of our team’s commitment to achieve the targets and aspirations of our business. We are looking forward to working with our clients and offering services from early stage development through to clinical manufacture. These are exciting times for Upperton as we continue to move forward and expand our capabilities.”

To learn more visit our GMP page .

About Upperton Pharma Solutions: At a time when the global CDMO market place is consolidating, Upperton Pharma Solutions remains as one of the few independent CDMO service providers with a specific focus on developing pharmaceutical dosage forms using its specialised spray drying capabilities. Upperton provides a range of integrated services to the pharmaceutical and biotech sectors around the world. The services include: early feasibility studies, formulation development for both small molecule APIs and Biotherapeutics for a range of final dosage forms and GMP manufacture of materials for Phase I & II clinical trials. Full analytical support, including ICH stability studies, is offered.

The company is a privately-owned organisation that has grown organically over almost 20 years and now employs 24 highly skilled personnel. Upperton Pharma Solutions has its headquarters in Nottingham, UK.

Contact:

Klara Fisken

Business Development Associate

Upperton Pharma Solutions, Albert Einstein Centre, Nottingham Science & Technology Park
Nottingham NG7 2TN
T: +44 (0) 115 855 7050 (Monday to Friday 8.30am – 4.30pm)

E: This email address is being protected from spambots. You need JavaScript enabled to view it. 

W: www.upperton.com



2nd November 2018 - Upperton Exhibits at AAPS PharmSci360

Upperton Pharma Solutions will be exhibiting at AAPS PharmSci360 for the first time, with this year’s event running from November 5th – 7th in Washington, D.C.

·         CEO & Founder Dr Richard Johnson and Business Development Executive Annette Kleiser will be available at booth 1643 to discuss how Upperton can support your development programme from early feasibility to clinical trials manufacturing

·         Richard will also be presenting a scientific poster titled “Developing Cold Chain Independent Vaccines - Spray-drying of Virosomes to Produce Dry Powder Formulations”, on Tuesday 6th November

·         This presentation will be based on the work of the MACIVIVA project which has the end goal of producing pioneering virosome-based vaccines against life threatening dise ases

Find Richard at Booth 1643 or arrange a meeting by contacting us.

26 September 2018 - Upperton Welcomes New Staff

Through extending our capabilities Upperton’s family have grown from three members of  staff four years ago, to twenty-one staff this year. The latest staff updates are:

·         Paul Kelsall, Director of Clinical Trials Manufacturing

Bringing experience in both sterile and non-sterile manufacturing, formulation development and facilities management. Paul formerly worked for Boots, Reckitt Benkiser, Nova Laboratories and Aesica.

·         Sarah Bayliss, Director of Quality & Compliance

Experience of implementing, maintaining and remediating quality management systems. Sarah joins us from Aesica.

·         Thomas Williams, Head of Clinical Manufacturing

Experience in developing and manufacturing investigational medicinal products for Phase I and II clinical trials. Thomas joins us from Quotient Sciences.

·         Rebecca Gray, Senior QC Analyst

Experience in QC testing, early formulation development analysis and analytical method qualification. Rebecca joins us from Quotient Sciences.

·         Klara Fisken, Business Development Associate

Klara joins us from the University of Nottingham, where she has just completed an MSc in Biopharmaceutical Biotechnology and Entrepreneurship.

·         Ella Rose, Junior Project Scientist

Ella joins us from Nottingham Trent University as a year long placement student, where she is currently studying towards a BSc in Chemistry.

19th September 2018 - Extended Pilot Plant Capabilities

 

Our newly expanded Pilot Plant has enabled us to provide our customers with a seamless transfer of processes from laboratory to clinical trials manufacture. In particular we offer three scales of spray dryer, all of which can also be operated in our GMP plant. Spray dryers in our pilot plant include:

·         ProCepT 4M8-TriX spray dryer

·         Intermediate spray dryer (Upperton design)

·         Niro Mobile Minor

These spray driers can spray dry a range of batch sizes (from 10mg to 3kg), allowing Upperton to conduct feasibility studies at scale to enable rapid transfer and reduce risk on scale up.

 

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With over 20 years of experience we have successfully spray dried, formulated and characterised a vast range of pharmaceutical and biotechnology products for companies across the globe.

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Upperton Pharma Solutions,
Albert Einstein Centre,
Nottingham Science & Technology Park,
Nottingham
NG7 2TN

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